Leucine 2,3-aminomutase, an enzyme of leucine catabolism.

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Leucine 2,3-aminomutase, an enzyme of leucine catabolism.

The initial step in the fermentation of leucine to acetate, isobutyrate, and ammonia by Clostridium sporogenes is the B12 coenzyme-dependent conversion of alpha-leucine to beta-leucine (3-amino-4-methylpentanoate). The amino group migration reaction, catalyzed by leucine 2,3-aminomutase, is reversible and is inhibited by intrinsic factor. The enzyme activity has been found in several clostridia...

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Leucine Zippers

The leucine zipper (ZIP) motif consists of a periodic repetition of a leucine residue at every seventh position and forms an a-helical conformation, which facilitates dimerization and in some cases higher oligomerization of proteins. Inmany eukaryotic gene regulatory proteins, the ZIP motif is flanked at its N-terminus by a basic region containing characteristic residues that facilitate DNA bin...

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Leucine Zipper

The leucine zipper is the dimerization domain of the B-ZIP (basic-region leucine zipper) class of eukaryotic transcription factors (Vinson et al., 1989). The name arose because leucines occur every seven amino acids in this dimerization domain.These leucines are critical for the dimerization and DNA binding of B-ZIP proteins. The leucine zipper is a left-handed parallel dimeric coiled-coil, a s...

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Leucine aminopeptidase fragments from an ascites tumor.

By 1946 (4) about 35 enzymes had been isolated and crystallized, and the controversy over their chemical nature was resolved with the conclusion that all enzymes contained a protein component essential for their activity. In 1951, however, Binkley (5-7) claimed to have purified highly active peptidases which analyzed as polynucleotides and were free from protein. His procedure was designed to e...

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Biosynthesis of leupeptin. III. Isolation and properties of an enzyme synthesizing acetyl-L-leucine.

An enzyme which catalyzes acetylation of L-leucine with acetyl-CoA was partially purified from a cell extract of Streptomyces roseus MA839-A1, a leupeptin producer. The molecular weight of this enzyme is about 27,000 daltons. The enzyme has fairly broad specificity for acyl donors (I) and acceptors (II): as for I, propionyl-CoA was 1/10 as active as acetyl-CoA with L-leucine as the acceptor; as...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1976

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(17)33627-x